GJMPR Volume 6, Number 1: January-June, 2017

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Determination of Bioactive Chemical Composition of Zingiber Officinale Essential Oil and Investigation of Its Anti-Microbial Activity

Hicham Boughendjioua

ABSTRACT Background: Ginger is known in many countries of the world for its culinary value, but also for its therapeutic virtues, widely marketed in the Algerian markets, we are interested in its chemical composition and its biological activities. Objective: The aim of this study was to determine the chemical composition of essential oil of Zingiber officinale rhizome and evaluate their antimicrobial activity. Results: The essential oil was obtained by hydrodistillation, and the chemical composition of the oil was determined by GC-MS. It was identified 17 compounds in the essential oil, the most abundant were; Zingiberene (30.80%), α-Curcumene (15.80%) and β-Sesquiphellandrene (15.56%). To determine the antimicrobial activity diffusion, agar dilution was employed. Conclusion: The results validate the medicinal use of Zingiber officinale in the treatment of diseases of possible infectious origin.

[ FULL TEXT PDF 1-5 ] DOI: 10.22587/gjmpr.2018.6.1.1

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Antibacterial Properties of Bambusa vulgaris (Bamboo) Leaves and Eryngium foetidum (Culantro) Leaves against Staphylococcus aureus and Escherichia coli bacteria

Marloie T. Menchavez*, Jerico D. Catipay, Aileen S. Espra, Pedrito M. Castillo II

ABSTRACT Staphylococcus aureus and Escherichia coli are considered to be two of the most prevalent gram-positive and gram-negative bacteria, and the most frequent sources of bacterial infections and hospital-acquired diseases worldwide (Bachir raho and Abouni, 2015). This investigation examines the antibacterial properties of Bambusa vulgaris (Bamboo) leaves and Eryngium foetidum (Culantro) leaves against the microbial growth of S. aureus and E. coli in vitro, and determines the phytochemical constituents present in the ethanol extracts that could be responsible for the said mechanism of action. Phytochemical Analysis and Antibacterial Test – Paper Disc Diffusion Method were the standardized laboratory methods used in the study. Based on the result of phytochemical screening, the identified phytochemicals present in Bambusa vulgaris ethanol extract were alkaloids, quaternary bases, amines, unsaturated steroids, and saponins. On the other hand, Eryngium foetidum ethanol extract contains alkaloids, quaternary bases, amines, 2 deoxysugars, unsaturated steroids, and tannins. The antibacterial activity of B. vulgaris and E. foetidum extracts were evaluated using Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative). Zone of inhibition was observed in vitro via paper disc diffusion method. B. vulgaris reached 26mm on the first trial, 27mm on the second and 26 mm on the third trial with an average of 26mm against S. aureus, whereas in inhibiting the growth of E. coli bacteria, it reached 20mm growth inhibition from trials 1 to 3 respectively. The extract from the E. foetidum (culantro) leaves grasped 22mm on the first trial and 23mm on the second and third trial with an average of 23mm against S. aureus, while in E. coli it exhibited 20 mm of zone inhibition from first to second trial and 21 mm on the third trial with an average of 20mm. The results of the tests on both samples implicate that these plants could potentially be used as antibacterial agents and on other medicinal applications. These findings could provide a prognostic approach in terms of addressing the manifestations caused by the foregoing detriments.

[ FULL TEXT PDF 6-13 ] DOI: 10.22587/gjmpr.2018.6.1.2

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